Whole Genome Sequencing (WGS) facility has been introduced in our laboratory. With exponentially decrease in cost, WGS is increasingly becoming the preferred technique for TB research. WGS is used to determine the complete DNA sequence of an organism's genome at a single time and can provide several answers at a single time, making it the ideal tool for studying the pathogen. Large-scale WGS has been applied to differentaspects of TB research; to study chains of transmission, disease outbreaks and also to infer phylogeny. Furthermore, WGS can be used to identify drug-resistance associated mutations and mutations compensating for the fitness defect associated with rifampicin resistance.